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recombinant human activin a  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human activin a
    Recombinant Human Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+activin+a/Recombinant+Human+Activin+AB+Protein/bio_rxiv__64898__2026__04__14__718527-142-7-11
    Average 94 stars, based on 54 article reviews
    recombinant human activin a - by Bioz Stars, 2026-09
    94/100 stars

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    Immunostaining:

    Article Title: Sox9 links biliary maturation to branching morphogenesis
    Article Snippet: .. Primary antibodies were used at the following concentrations for immunostaining: 2.5μg/mL anti-Activin A (R&D Systems; MAB3381), 0.75μg/mL anti-pSMAD2 (Ser465/467) (Cell Signaling; 3108T), 2.5μg/mL anti-EpCAM/CD326 (BioLegend; 118201), 1μg/mL anti-Ki-67 (Invitrogen; PA5-19462), 7μg/mL anti-Ac-alpha-Tubulin (K40), 0.16μg/mL anti-Cleaved Caspase 3 (D175) (Cell Signaling; 5335S), 0.2μg/mL anti-NCAM1 (Cell Signaling; 99746T), 1μg/mL anti-SOX9 (Millipore; AB5535), 2.5μg/mL anti-EpCAM/CD326-647 (BioLegend; 118212). .. Secondary antibodies used for immunostaining: 4μg/mL donkey anti-rabbit Alexa Fluor 555 (ThermoFisher; A31572), 4μg/mL donkey anti-rat Alexa Fluor 488 (ThermoFisher; A21208), 4μg/mL donkey anti-mouse Alexa Fluor 555 (ThermoFisher; A31570), and 5μg/mL goat anti-rabbit HRP (ThermoFisher; G21234).

    Article Title: Sox9 inhibits Activin A to promote biliary maturation and branching morphogenesis.
    Article Snippet: .. Primary antibodies were used at the following concentrations for immunostaining: 2.5μg/mL anti-Activin A (R&D Systems; MAB3381), 0.75μg/mL anti-pSMAD2 (Ser465/467) (Cell Signaling; 3108 T), 2.5μg/ mL anti-EpCAM/CD326 (BioLegend; 118201), 1 μg/mL anti-Ki-67 (Invitrogen; PA5-19462), 7μg/mL anti-Ac-alpha-Tubulin (K40)(Cell Signaling; 5335), 0.16μg/mL anti-Cleaved Caspase 3 (D175) (Cell Signaling; 9661), 0.2μg/mL anti-NCAM1 (Cell Signaling; 99746T), 5μg/mL antiOPN/SPP1 (R&D; AF808), 1μg/mL anti-SOX9 (Millipore; AB5535), 2.5μg/mL anti-EpCAM/CD326-647 (BioLegend; 118212). .. Secondary antibodies used for immunostaining: 4μg/mLdonkey anti-rabbit Alexa Fluor 555 (ThermoFisher; A31572), 4μg/mL donkey anti-rat Alexa Fluor 488 (ThermoFisher; A21208), 4μg/mL donkey anti-mouse Alexa Fluor 555 (ThermoFisher; A31570), and 5μg/mL goat anti-rabbit HRP (ThermoFisher; G21234).

    Article Title: Sox9 inhibits Activin A to promote biliary maturation and branching morphogenesis
    Article Snippet: .. Primary antibodies were used at the following concentrations for immunostaining: 2.5 μg/mL anti-Activin A (R&D Systems; MAB3381), 0.75 μg/mL anti-pSMAD2 (Ser465/467) (Cell Signaling; 3108 T), 2.5 μg/mL anti-EpCAM/CD326 (BioLegend; 118201), 1 μg/mL anti-Ki-67 (Invitrogen; PA5-19462), 7 μg/mL anti-Ac-alpha-Tubulin (K40)(Cell Signaling; 5335), 0.16 μg/mL anti-Cleaved Caspase 3 (D175) (Cell Signaling; 9661), 0.2 μg/mL anti-NCAM1 (Cell Signaling; 99746 T), 5 μg/mL anti-OPN/SPP1 (R&D; AF808), 1 μg/mL anti-SOX9 (Millipore; AB5535), 2.5 μg/mL anti-EpCAM/CD326-647 (BioLegend; 118212). .. Secondary antibodies used for immunostaining: 4 μg/mL donkey anti-rabbit Alexa Fluor 555 (ThermoFisher; A31572), 4 μg/mL donkey anti-rat Alexa Fluor 488 (ThermoFisher; A21208), 4 μg/mL donkey anti-mouse Alexa Fluor 555 (ThermoFisher; A31570), and 5 μg/mL goat anti-rabbit HRP (ThermoFisher; G21234).

    Incubation:

    Article Title: ID1 high /activin A high glioblastoma cells contribute to resistance to anti-angiogenesis therapy through malformed vasculature
    Article Snippet: .. These sections were blocked with 3% BSA containing 0.3% Triton X-100 in PBS overnight at 4°C and incubated with the primary anti-CD31 (1:200; Merck, NJ, USA, Cat. MAB1398Z), anti-Activin A (1:50; R&D Systems, Cat. AF338), anti-Slug (1:100; Cell Signaling, Cat. 9585), anti-PDGFRβ (1:50; eBioscience, CA, USA, Cat. 14-1402-82), anti-cleaved CASP3 (1:200; Cell Signaling Cat. 9661), and anti-VEGFR2 (1:200; Cell Signaling, Cat. 2479) antibodies for 12 h at 4°C. .. After washing four times with PBS containing 0.1% Tween-20 (PBS-T), the sections were incubated with the Alexa Fluor 488-conjugated, Alexa Fluor 568-conjugated, or Alexa Fluor 647- conjugated secondary antibodies (1:400; Jackson Lab, Cat. 127-605-160) for 12 h at 4°C.

    Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis
    Article Snippet: Slides were washed in 0.1 M PBS and blocked with SeaBlock (Thermo Scientific; Rockford, IL, USA; cat. #37527) for 1 h at room temperature. .. Slides were then incubated with primary antibodies (diluted in 0.1% Triton‐X in 0.1 M PBS, 10% SeaBlock [overnight at 4°C]): goat anti‐activin‐A (R&D Systems #AF338; 1:25), rabbit anti‐βIII‐Tubulin (Sigma‐Aldrich #T2200; 1:500), rat anti‐ED‐1/CD68 (Abcam #ab53444; 1:500), rabbit anti‐ED‐1/CD68 (Abcam #ab125212; 1:600), rabbit anti‐inducible nitric oxide synthase (iNOS; Sigma‐Aldrich #N7782; 1:500), rat anti‐myelin basic protein (MBP; NovusBio #NB600‐717; 1:20), goat anti‐platelet derived growth factor receptor alpha (PDGFRα; R&D Systems #AF1062; 1:75), mouse anti‐SMI31 (Cedarlane #SMI‐31R; 1:1000). .. After rinsing with 0.1 M PBS, secondary antibodies (diluted in 0.01% Triton‐X in 0.1 M PBS, 1% SeaBlock [1 h room temperature]) were applied: goat anti‐mouse Alexa488 (Abcam #150117; 1:1000), mouse anti‐rat Alexa488 (Jackson ImmunoResearch #212‐545‐106; 1:1000), donkey anti‐rabbit CY3 (Jackson ImmunoResearch #711‐166‐152; 1:3000), donkey anti‐goat DyLight488 (Jackson ImmunoResearch #705‐485‐003; 1:1000).

    Mouse Assay:

    Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia
    Article Snippet: .. Mice aged 10–14 weeks were treated systemically with anti-activin A neutralizing antibody (R&D systems, MAB3381; 10 mg kg −1 body weight) 1 week after tumor implantation through the tail vein every 4 days. .. The same dose of IgG1 isotype control (R&D systems, MAB002) was used as the control antibody.

    Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia.
    Article Snippet: .. Mice aged 10–14 weeks were treated systemically with anti-activin A neutralizing antibody (R&D systems, MAB3381; 10 mg kg−1 body weight) 1 week after tumor implantation through the tail vein every 4 days. .. The same dose of IgG1 isotype control (R&D systems, MAB002) was used as the control antibody.

    Tumor Implantation:

    Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia
    Article Snippet: .. Mice aged 10–14 weeks were treated systemically with anti-activin A neutralizing antibody (R&D systems, MAB3381; 10 mg kg −1 body weight) 1 week after tumor implantation through the tail vein every 4 days. .. The same dose of IgG1 isotype control (R&D systems, MAB002) was used as the control antibody.

    Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia.
    Article Snippet: .. Mice aged 10–14 weeks were treated systemically with anti-activin A neutralizing antibody (R&D systems, MAB3381; 10 mg kg−1 body weight) 1 week after tumor implantation through the tail vein every 4 days. .. The same dose of IgG1 isotype control (R&D systems, MAB002) was used as the control antibody.

    Derivative Assay:

    Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis
    Article Snippet: Slides were washed in 0.1 M PBS and blocked with SeaBlock (Thermo Scientific; Rockford, IL, USA; cat. #37527) for 1 h at room temperature. .. Slides were then incubated with primary antibodies (diluted in 0.1% Triton‐X in 0.1 M PBS, 10% SeaBlock [overnight at 4°C]): goat anti‐activin‐A (R&D Systems #AF338; 1:25), rabbit anti‐βIII‐Tubulin (Sigma‐Aldrich #T2200; 1:500), rat anti‐ED‐1/CD68 (Abcam #ab53444; 1:500), rabbit anti‐ED‐1/CD68 (Abcam #ab125212; 1:600), rabbit anti‐inducible nitric oxide synthase (iNOS; Sigma‐Aldrich #N7782; 1:500), rat anti‐myelin basic protein (MBP; NovusBio #NB600‐717; 1:20), goat anti‐platelet derived growth factor receptor alpha (PDGFRα; R&D Systems #AF1062; 1:75), mouse anti‐SMI31 (Cedarlane #SMI‐31R; 1:1000). .. After rinsing with 0.1 M PBS, secondary antibodies (diluted in 0.01% Triton‐X in 0.1 M PBS, 1% SeaBlock [1 h room temperature]) were applied: goat anti‐mouse Alexa488 (Abcam #150117; 1:1000), mouse anti‐rat Alexa488 (Jackson ImmunoResearch #212‐545‐106; 1:1000), donkey anti‐rabbit CY3 (Jackson ImmunoResearch #711‐166‐152; 1:3000), donkey anti‐goat DyLight488 (Jackson ImmunoResearch #705‐485‐003; 1:1000).



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